-TCR immunobank analysis

TCR-β Repertoire Quantitative Analysis
The T-cell receptor (TCR) serves as a pivotal molecule in T-cell antigen recognition and is fundamental to adaptive immune responses. Its diversity represents a critical biomarker for assessing immune function.
InnoStar's TCR-β repertoire sequencing technology overcomes the constraints of RNA-level sequencing by targeting DNA-level analysis, ensuring highly accurate TCR clonotype quantification. This advanced approach supports precision immune monitoring, tumor immunology research, and investigations into cellular immunotherapy, offering valuable insights into disease progression and therapeutic efficacy. Furthermore, it allows for the tracking of temporal dynamics and quantitative changes in challenging-to-monitor cellular therapies, including tumor-infiltrating lymphocytes (TILs).

Elaborately Design for Highly Accurate Quantitative Analysis
The human immune system contains an estimated 10¹³ unique TCR clonotypes, generated through V(D)J recombination during T-cell development. The hypervariable CDR3 region within the TCRβ chain serves as the key determinant of antigen specificity, offering unparalleled diversity for immune recognition.
InnoStar’s TCR-β immune repertoire sequencing employs a highly multiplexed PCR-based approach, leveraging 60+ optimized primers for comprehensive V(D)J coverage. By using genomic DNA as input, the method ensures targeted CDR3 enrichment while minimizing amplification bias. Coupled with a proprietary bioinformatic correction algorithm, this technology achieves ultra-precise clonotype quantification and reliable assessment of TCR diversity.
Key features include:
-Optimized primer pool for maximal sequence coverage
-Bias-correction computational model for enhanced accuracy
-Superior precision and linearity, even at low clonotype frequencies
-High batch-to-batch reproducibility for consistent results
-Comprehensive coverage, capable of profiling all currently known TCR subtypes

Common Applications
•    Assessment of Immune Complexity: Evaluates individual TCR diversity to reveal immune system status.
•    Unmodified TILs Therapy Quantification: Measures TILs dynamics in tumor immunotherapy to assess drug efficacy.
•    Immune Therapy Monitoring: Tracks TCR clonotype changes before and after treatment at different time points, supporting personalized therapeutic strategies.